Part:BBa_K766000:Design

AHL synthetase LuxI with His-tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We want to find a way to detect the expression and purify LuxI protein.

Source

This part is from BBa_C0261. We make a truncation on the C-terminal to cut off the LVA tag. His tag and thrombin cutting site is add on the N-terminal.

Thank robertb from MIT!